Journal: Journal for Immunotherapy of Cancer

Article Title: An anti-mesothelin targeting antibody drug conjugate induces pyroptosis and ignites antitumor immunity in mouse models of cancer

doi: 10.1136/jitc-2022-006274

Figure Lengend Snippet: MesoADC shows reduced effectiveness in GSDME KO tumors, which is restored when combined with Flt3L. Mice bearing EMT6 CRISPR control or GSDME CRISPR KO tumors were treated with vehicle control or mesoADC at 4.4 mg/kg alone or with 10 µg hFlt3L/day for 10 days. Mice were monitored for 45 days. Data are presented as Kaplan-Meier survival curves, n=6–8. (A) Comparison of survival for mice bearing CRISPR control and GSDME CRISPR KO tumors. (B) Comparison of CRISPR control+ADC and GSDME CRISPR KO+ADC. (C) Comparison of GSDME CRISPR KO with ADC and Flt3L alone, and in combination. Pairwise comparison was performed for each treatment compared with GSDME CRISPR KO. (D) Summary of median survival and complete response for each treatment group. *P<0.05, **P<0.01, ***P<0.01. ADC, antibody drug conjugate; Flt3L, Fms-like tyrosine kinase-3 ligand; GSDME, gasdermin E; KO, knockout.

Article Snippet: Recombinant human Flt3L was purchased from Sino Biological.

Techniques: CRISPR, Knock-Out

Journal: Leukemia

Article Title: Selective apoptosis of tandemly duplicated FLT3-transformed leukemia cells by Hsp90 inhibitors.

doi: 10.1038/sj.leu.2402558

Figure Lengend Snippet: Figure 3 (a) Dephosphorylation of TDFLT3 by HA. TDFLT3/32D and WtFLT3/32D (with 50 ng/ml FL) were treated with HA at 0.1, 0.3 and 1.0 M for 12 h. Cell lysates were immunoprecipitated by anti- FLT3 antibody and immunoblotted by anti-phosphotyrosine antibody or by anti-FLT3 antibody. Whole cell lysates were also immunoblotted by anti-FLT3 antibody and anti-Actin antibody for loading control. (b) Dephosphorylation of MAPK, Akt and STAT5a in MtFLT3/32D treated with HA. Cells were cultured with or without 0.3 MHA and lysed after 12 h. Whole cell lysates were immunoblotted by anti- MAPK and phospho-MAPK antibodies. Cell lysates were immunoprec- ipitated by anti-Akt or by anti-STAT5a antibodies, and immunoblotted by indicated antibodies.

Article Snippet: Recombinant human FLT3 ligand (FL) was purchased from R&D Systems (Minneapolis,

Techniques: De-Phosphorylation Assay, Immunoprecipitation, Control, Cell Culture

Journal: Leukemia

Article Title: Selective apoptosis of tandemly duplicated FLT3-transformed leukemia cells by Hsp90 inhibitors.

doi: 10.1038/sj.leu.2402558

Figure Lengend Snippet: Figure 4 Association of FLT3 with Hsp90. WtFLT3/32D stimulated with or without 50 ng/ml FL for 10 min were analyzed by immunopre- cipitation followed by immunoblotting using indicated antibodies. TDFLT3/32D was treated with HA at 0.03, 0.1 and 0.3 M HA for 12 h and was assayed. For detection of Hsp90 expression levels, whole cell lysates were immunoblotted by anti-Hsp90 antibody.

Article Snippet: Recombinant human FLT3 ligand (FL) was purchased from R&D Systems (Minneapolis,

Techniques: Western Blot, Expressing